# Metadata for Genome-Wide CRISPR Screen Organism: Homo sapiens Cell line: RPMI-8226 (ATCC¨ CRL-3216ª) Cas9: Stably expressing SpCas9 (Addgene plasmid #52962) Library: Brunello Human CRISPR Knockout Pooled Library (Addgene #73178) MOI (multiplicity of infection): 0.2 Antibiotic selection: Puromycin, 2 µg/ml, 4 days Selection marker: Endogenous EGFP-tagged MYC Number of sgRNAs: ~76,441 Number of genes targeted: ~19,000 Number of replicates: 3 biological replicates per condition (sorted low [5%], sorted intermediate [90%], sorted high [5%]) Sorting strategy: FACS based on EGFP fluorescence intensity Sequencing platform: Illumina NovaSeq 6000 Data analysis software: MAGeCK MLE Controls: Non-targeting sgRNAs included Responsible PI: Frank SchnŸtgen Contact: schnuetgen@em.uni-frankfurt.de Date of experiment: 02/2022 Location: Goethe University Frankfurt, University Medicine Frankfurt, Department of Medicine Hematology/Oncology, Frankfurt/Main 60590, Germany